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Abstract

Background: Ultraviolet B exposure is one of the causes of extrinsic skin aging. Ultraviolet exposure may lead to skin collagen damage. This study about mesenchymal stem cells from umbilical cord (UCMSC) compared with platelet rich plasma (PRP) with the aim was to prove that the intradermal injection of UCMSC was more effective than PRP in increasing amount of collagen and fibroblasts in Wistar rats' back skin exposed to Ultraviolet B rays.


Methods: This study used post-test only control group design. Subjects were 36 Wistar rats aged 2.5 months. A control group consisting of 18 Wistar rats treated with intradermal injection of PRP and the treatment group with UCMSC. Both groups were exposed to UVB with a total radiation dose of 840 mJ/cm2. The parameter of collagen was determined by picro sirius red staining while fibroblasts by Haematoxylin-Eosin staining.


Result: The mean amount of collagen in the control group was lower (57,82±6.52%) than the treatment group (65.69±4.51%) with p<0.001. The result showed there were differences in the number of collagen in both groups (p<0.05). The mean number of fibroblasts in the control group was lower (22.63±5.98 cell/field of view) than the treatment group (28.20±8.90 cell/field of view) with p=0.034. The result showed there were differences in mean of fibroblasts in both groups (p<0.05).


Conclusion: It can be concluded that the intradermal injection of UCMSC was more effective in increasing the amount of collagen and fibroblasts than intradermal injection of PRP in Wistar rats' back skin exposed to UVB rays.

Keywords

Umbilical cord mesenchymal stem cell (UCMSC) Platelet rich plasma (PRP) UVB rays Collagen Fibroblasts

Article Details

How to Cite
Kencanawati, A. C., Pangkahila, W., & Wiraguna, A. A. G. P. (2021). Intradermal injection of umbilical cord mesenchymal stem cell was more effective than platelet rich plasma in increasing amount of collagen and fibroblasts in Wistar rats (Rattus norvegicus) back skin exposed to ultraviolet B rays. Neurologico Spinale Medico Chirurgico, 4(1), 42-46. https://doi.org/10.36444/nsmc.v4i1.152

References

  1. Pangkahila W. Anti aging medicine: Tetap muda dan sehat. 1st ed. Jakarta: Kompas Media; 2011.
  2. Uito J, Chu M, Gallo R, et al. Collagen, elastic fibers and extracellular matrix of the dermis. New York: McGraw-Hill; 2011:2560-2582.
  3. Arutyunyan I, Elchaninov A, Makarov A, et al. Umbilical Cord as prospective source for mesenchymal stem cell-based therapy. Stem Cells International. 2016;1(1):1-17. DOI: 10.1155/2016/6901286
  4. Marx R. Platelet Rich Plasma (PRP). Implant Dentistry. 2011;10(4):22.
  5. Federer W. Statistics and society, data collection and interpretation. 2nd ed. New York: Marcel Dekker; 2011.
  6. Kim YJ, Seo DH, Lee SH, et al. Conditioned media from human umbilical cord blood-derived mesenchymal stem cells stimulate rejuvenation function in human skin. Biochemistry and Biophysics Reports. 2018;16:96-102. DOI: 10.1016/j.bbrep.2018.10.007
  7. Lee EJ, Kim GH, Kang SK, et al. Regeneration of peripheral by transplanted sphere of human mesenchymal stem cells derived from embryonic stem cells. Biomaterials. 2012; 33(29):7039-7046. DOI: 10.1016/j.biomaterials.2012.06.047
  8. Hartono B. Sel punca: Karakteristik, potensi, dan aplikasi. Jurnal Kedokteran Meditek. 2016;22(60):1456.
  9. Syaifudin M. Pemberian sel punca CD34 darah tepi manusia secara subkutan meningkatkan jumlah sel fibroblas dan kolagen pada tikus jantan Wistar yang dipajan sinar ultraviolet B [thesis]. Denpasar: Program Magister Biomedik Universitas Udayana; 2015.
  10. Suhalim LTR. Pemberian kombinasi membran amnion kering dengan platelet rich plasma meningkatkan neovaskularisasi dan fibroblas lebih banyak serta epitelisasi lebih tebal daripada membran amnion kering pada tikus (Rattus norvegicus) jantan Galur Wistar yang dilukai [thesis]. Denpasar: Program Magister Biomedik Universitas Udayana; 2019.
  11. Chandra IA. Pemberian gel nanochitosan-PRP topikal menurunkan ekspresi MMP-1 dan meningkatkan jumlah kolagen pada jaringan luka tikus Wistar [thesis]. Denpasar: Program Magister Biomedik Universitas Udayana; 2014.