Main Article Content
Background: Centella asiatica has been popularly used in cosmetic products, especially due to its antioxidant benefits in soothing and healing wounds. However, the inhibition of melanogenesis in the content of Centella asiatica has not been widely studied. This study aimed to prove the effectiveness of 10% Centella asiatica cream on male Guinea pig (Cavia porcellus) skin exposed to UVB.
Methods: The randomized posttest-only control group study was conducted on 36 Guinea pigs aged 3-months, body weight 300-350 grams with a healthy skin condition. They were randomly divided into two groups; each consists of 18 Guinea pigs. The first group was a control group given base cream 20-minutes before and 4-hours after UVB exposure, 3-times-a-week for two weeks. The second group was the treatment group given 10% Centella asiatica cream and expose to UVB with the same procedure. The histopathological preparations were made using a punch biopsy after intervention was completed. Immunohistochemical staining was performed to measure the expression of microphthalmia-associated-transcription-factor (MITF) and Masson-Fontana to measure the amount of melanin. Data analysis was conducted to compare the results in both groups.
Results: The treatment group had a significantly lower mean MITF expression (13.61 ± 5.36 vs. 27.24 ± 6.50%) and melanin amount (5.95 ± 3.81 vs. 16.59 ± 5.77%) (p < 0.001) compared to the control group.
Conclusion: The administration of cream consists of Centella asiatica extract 10% effectively inhibits the expression of MITF and prevented the increase of melanin amount in male guinea pigs (Cavia porcellus) exposed to UVB.
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Wiraguna AAGP, Hari ED, Praharsini IGAA. Correlation between glutathione plasma with degree severity of melasma in Balinese women. Clin Cosmet Investig Dermatol. 2020;13:455–459. DOI: 10.2147/CCID.S258834
- D’Mello SAN, Finlay GJ, Baguley BC, et al. Signaling pathways in melanogenesis. Int J Mol Sci. 2016;17:E1144. DOI: 10.3390/ijms17071144
- Katiyar S, Yadav D. Correlation of oxidative stress with melasma: An overview. Curr Pharm Des. 2021 Nov 4. DOI: 10.2174/1381612827666211104154928.
- Panzella L, Napolitano A. Natural and bioinspired phenolic compounds as tyrosinase inhibitors for the treatment of skin hyperpigmentation: Recent advances. Cosmetics. 2019;6(4):57. DOI: 10.3390/cosmetics6040057
- Lu H, Yang K, Zhan L, et al. Optimization of flavonoid extraction in Dendrobium officinale leaves and their inhibitory effects on tyrosinase activity. Int J Anal Chem. 2019;2019:7849198. DOI: 10.1155/2019/7849198
- Seevaratnam V, Banumathi P, Premalatha MR, et al. Functional properties of Centella asiatica (L.): A review. Int J Pharm Pharm Sci. 2012;4:8–14.
- Siahaan ER, Pangkahila W, Wiraguna A. Krim ekstrak kulit delima merah (Punica granatum) menghambat peningkatan jumlah melanin sama efektifnya dengan krim hidrokuinon pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB. JURNAL BIOMEDIK: JBM. 2017;9(1). DOI: 10.35790/jbm.9.1.2017.15313
- Merysia M, Pangkahila W. Cream of leaf pare extract (Momordica caharantia l) 4% takes the improvement of effective melanin numbers with 4% hydrocuinon cream on marmut skin (Cavia porcellus) heart which uses UVB ultraviolet light. IJAAM (Indonesian Journal of Anti-Aging Medicine). 2018;2:24–27.
- Lee TH, Seo JO, Baek S-H, et al. Inhibitory effects of resveratrol on melanin synthesis in ultraviolet B-induced pigmentation in Guinea pig skin. Biomol Ther (Seoul). 2014;22:35–40. DOI: 10.4062/biomolther.2013.081
- Saraf S, Chhabra SK, Kaur CD, et al. Development of photochemoprotective herbs containing cosmetic formulations for improving skin properties. J Cosmet Sci. 2012;63:119–131.
- Kwon KJ, Bae S, Kim K, et al. Asiaticoside, a component of Centella asiatica, inhibits melanogenesis in B16F10 mouse melanoma. Molecular medicine reports. 2014;10:503–507. DOI: 10.3892/mmr.2014.2159
- Akmal M, Adam M, Toras M, et al. Pengaruh pemberian ekstrak daun pegagan (Centella asiatica (l.) urban) terhadap konsentrasi testosteron pada tikus putih jantan (rattus norvegicus). Jurnal Medika Veterinaria. 2015;9(1). DOI: 10.21157/j.med.vet..v9i1.2993
- Srichaiyo N, Tongsiri S, Hoseinifar SH, et al. The effects gotu kola (Centella asiatica) powder on growth performance, skin mucus, and serum immunity of Nile tilapia (Oreochromis niloticus) fingerlings. Aquaculture Reports. 2020;16:100239. DOI: 10.1016/j.aqrep.2019.100239
- Fan M, Ding H, Zhang G, et al. Relationships of dietary flavonoid structure with its tyrosinase inhibitory activity and affinity. LWT. 2019;107:25–34.
- Şöhretoğlu D, Sari S, Barut B, et al. Tyrosinase inhibition by some flavonoids: Inhibitory activity, mechanism by in vitro and in silico studies. Bioorg Chem. 2018;81:168–174. DOI: 10.1016/j.bioorg.2018.08.020
- Guo C, Shan Y, Yang Z, et al. Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel. J Sci Food Agric. 2020;100:2664–2674. DOI: 10.1002/jsfa.10297
- Erdogan Orhan I, Tareq Hassan Khan M. Flavonoid derivatives as potent tyrosinase inhibitors–a survey of recent findings between 2008-2013. Current topics in medicinal chemistry. 2014;14:1486–1493. DOI: 10.2174/1568026614666140523120741
- Promden W, Viriyabancha W, Monthakantirat O, et al. Correlation between the potency of flavonoids on mushroom tyrosinase inhibitory activity and melanin synthesis in melanocytes. Molecules. 2018;23:E1403. DOI: 10.3390/molecules23061403
- Pillaiyar T, Manickam M, Namasivayam V. Skin whitening agents: Medicinal chemistry perspective of tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2017;32:403–425. DOI: 10.1080/14756366.2016.1256882