Main Article Content

Abstract

Background: Exposure to cigarette smoke and UV-B, can activate enzymes that degrade collagen and inhibit its production through increased levels of MMP-1. Bidara leaves with high content of flavonoids and tannins may prevent damage caused by free radicals. This study aimed to prove the effect of bidara leaf extract in inhibiting the increase in MMP-1 levels and collagen reduction in female Wistar rats skin exposed to UV-B and cigarette smoke.


Methods: A randomized posttest-only control group design study was conducted on 30 female Wistar rats, aged 8-10 weeks, weighing 130-150 grams. The rats were divided into three groups: the group without treatment, the base cream, and the 7.5% bidara leaves extract groups. The last two groups were exposed to UV-B light and cigarette smoke. After four weeks of intervention, the back skin tissue was taken to assess collagen amount and MMP-1 levels. Comparative analysis was performed to see the difference between groups.


Results: The results showed that the mean levels of MMP-1 in the untreated group were lowest, followed by the bidara leaf extract cream and the base cream group. The mean of collagen amount of bidara leaf extract group was the highest, followed by the base cream and the untreated group. The analysis showed a significant difference in the mean levels of MMP-1 and collagen amount between groups (p<0.05).


Conclusion: The 7.5% bidara leaf extract cream inhibited the increase in MMP-1 levels and the decrease in collagen in female Wistar rats exposed to UV-B light and cigarette smoke.

Keywords

Anti-aging Animal study Bidara leaf Collagen MMP-1 Ultraviolet

Article Details

How to Cite
Anggraeny, C., Wiraguna, A. A. G. P., & Pinatih, G. N. I. (2021). Bidara leaf extract (Ziziphus mauritiana) inhibits MMP-1 increase and collagen decrease in female Wistar rat skin exposed to UVB and cigarette smoke. Neurologico Spinale Medico Chirurgico, 4(3), 125-129. https://doi.org/10.36444/nsmc.v4i3.178

References

  1. Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract. 2012;2012. DOI: 10.1155/2012/135206
  2. Rinnerthaler M, Bischof J, Streubel MK, et al. Oxidative stress in aging human skin. Biomolecules. 2015;5(2):545-589. DOI: 10.3390/biom5020545
  3. Krutmann J, Schikowski T, Morita A, et al. Environmentally-induced (extrinsic) skin aging: Exposomal factors and underlying mechanisms. J Invest Dermatol. 2021;141(4):1096-1103. DOI: 10.1016/j.jid.2020.12.011
  4. Krutmann J, Bouloc A, Sore G, et al. The skin aging exposome. J Dermatol Sci. 2017;85(3):152-161. DOI: 10.1016/j.jdermsci.2016.09.015
  5. Fisher GJ, Quan T, Purohit T, et al. Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin. Am J Pathol. 2009;174(1):101-114. DOI: 10.2353/ajpath.2009.080599
  6. Kusriani RH, Machter E. Penetapan kadar senyawa fenolat total dan aktivitas antioksidan ekstrak daun, buah dan biji bidara (ziziphus spina-christi l.). Pros SNaPP Kesehat Kedokt Kebidanan Keperawatan Farm Psikol. 2015;1(1):311-318.
  7. Mukhlishoh F, Verdianti P, Krihariyani D. The resistance profile of bacteria salmonella sp towards antibacterial ethanol extract of bidara leaves (Ziziphus mauritiana). 2018;381-385. DOI: 10.5220/0007543403810385
  8. Yacobus AR, Ghari ALS. Identifikasi senyawa kimia daun bidara (Ziziphus mauritiana Lam) dari Kabupaten Timor Tengah Selatan Provinsi NTT secara kromatografi lapis tipis dan kromatografi kolom. J Farm Sandi Karsa. 2018;4(7):5-10.
  9. Kaurinovic B, Vastag D. Flavonoids and phenolic acids as potential natural antioxidants. In: Antioxidants. IntechOpen London, UK; 2019:1-20.
  10. Dzia\lo M, Mierziak J, Korzun U, et al. The potential of plant phenolics in prevention and therapy of skin disorders. Int J Mol Sci. 2016;17(2):160. DOI: 10.3390/ijms17020160
  11. Satué K, Montesinos P, Muños A. Activation of the renin–angiotensin–aldosterone system in mares around ovulation. Czech J Anim Sci. 2017;62(6):259-267. DOI: 10.17221/32/2016-CJAS
  12. Zahruddin A, Damayanti D. Penuaan kulit: Patofisiologi dan manifestasi klinis. Berk Ilmu Kesehat Kulit Dan Kelamin. 2018;30(3):208-215. DOI: 10.20473/bikk.V30.3.2018.208-215
  13. Wiraguna AAGP. Aspek anataomi dan fisiologi kulit. 1st Edition. Udayana University, Bali; 2020.
  14. Surbakti BR. Perbandingan kadar kelembapan kulit pada wanita pra-menopause dengan wanita pasca-menopause di perkebunan kelapa sawit area II PT. Salim Ivomas Pratama KM 29, Bagan Batu, Riau. [Skripsi]. Medan: Universitas HKBP Nommensen. 2019.
  15. Liliana N, Wiraguna AAGP, Pangkahila W. Krim ekstrak Panax ginseng menghambat peningkatan ekspresi MMP-1 dan penurunan jumlah kolagen pada tikus Wistar jantan (Rattus norvegicus) yang dipajan sinar UV-B. eBiomedik. 2017;5(1). DOI: 10.35790/ebm.v5i1.15038
  16. Carrer V, Guzmán B, Martí M, et al. Lanolin-based synthetic membranes as percutaneous absorption models for transdermal drug delivery. Pharmaceutics. 2018;10(3):73. DOI: 10.3390/pharmaceutics10030073
  17. Sizeland KH, Wells HC, Kelly SJR, et al. The influence of water, lanolin, urea, proline, paraffin and fatliquor on collagen D-spacing in leather. RSC Adv. 2017;7(64):40658-40663. DOI: 10.1039/C7RA05560A
  18. Kadji MH, Runtuwene MR, Citraningtyas G. Uji fitokimia dan aktivitas antioksidan dari ekstrak etanol daun soyogik (Saurauia bracteosa DC). Pharmacon. 2013;2(2). DOI: 10.35799/pha.2.2013.1415
  19. Haryanto A, Pangkahila W, Wiraguna AAGP. Black rice bran (Oryza sativa l. indica) extract cream prevented the increase of dermal matrix metalloproteinase-1 and dermal collagen reduction of male Wistar rats (Rattus norvegicus) exposed to ultraviolet-B rays. IJAAM Indones J Anti-Aging Med. 2020;4(1):16-19. DOI: 10.36675/ijaam.v4i1.48
  20. Kuo Y-H, Lin T-Y, You Y-J, et al. Antiinflammatory and antiphotodamaging effects of ergostatrien-3β-ol, isolated from Antrodia camphorata, on hairless mouse skin. Molecules. 2016;21(9):1213. DOI: 10.3390/molecules21091213