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Background: Photoaging increases MMP-1 and damage collagen by forming ROS. The antioxidant compounds in Clitoria ternatea L. have been studied to lower ROS, thus inhibiting the emergence of MMP-1 and preserving the collagen. The study initiated to analyze the Clitoria ternatea L. extract cream 5% effect on inhibiting MMP-1 increase and collagen decrease in female Wistar rat's skin exposed to UV-B.

Methods: A randomized post-test-only control group design was conducted on 30 female Wistar rats, aged 8-10 weeks old with 130-150 grams bodyweight. They were assigned to three groups: the untreated group, placebo group, and the Clitoria ternatea L. extract cream 5% group. The last two groups were exposed to UV-B. After  four weeks their skin was examined for MMP-1 levels using ELISA and collagen amount using Picro Sirius Red staining.

Results: A comparative analysis showed that the lowest mean levels of MMP-1 was seen in the untreated group (4.64 ± 0.14 ng/ml), followed by the Clitoria ternatea L. extract cream group 5% (5.54 ± 0.21 ng/ml) and placebo group (8.68 ± 0.78 ng/ml). The highest mean of collagen amount was seen in the Clitoria ternatea L. extract cream group 5% (76.12 ± 5.09 %pixel), followed by the untreated group (69.38 ± 3.96 %pixel) and placebo group (30.31±8.06 %pixel). The comparative analysis shows a significant difference between groups (p < 0.05).

Conclusion: The Clitoria ternatea L. extract cream 5% has proven to inhibited the increase of MMP-1 levels and decrease of collagen amount in Wistar rats skin exposed to UV-B.


Anti-aging Clitoria ternatea Matrix mettaloprotein-1 Collagen Photoaging Ultraviolet

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How to Cite
Saritani, A. T. B., Wiraguna, A. A. G. P., & Maker, L. P. I. I. (2021). Clitoria ternatea L. extract cream 5% inhibited the increase of MMP-1 levels and decrease of collagen amount in wistar rats (Rattus norvegicus) dermic skin exposed to ultraviolet B. Neurologico Spinale Medico Chirurgico, 4(3).


  1. Kammeyer A, Luiten RM. Oxidation events and skin aging. Ageing Res Rev. 2015;21:16–29. DOI: 10.1016/j.arr.2015.01.001
  2. Addor FAS. Antioxidants in dermatology. An Bras Dermatol. 2017;92:356–62. DOI: 10.1590/abd1806-4841.20175697
  3. Bjørklund G, Chirumbolo S. Role of oxidative stress and antioxidants in daily nutrition and human health. Nutrition. 2017;33:311–21. DOI: 10.1016/j.nut.2016.07.018
  4. Cahyaningsih E, Yuda PESK, Santoso P. Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol bunga telang (Clitoria ternatea L.) dengan metode spektrofotometri uv-Vis. J Ilm Medicam. 2019;5:51–7. DOI: 10.36733/medicamento.v5i1.851
  5. Karak P. Biological activities of flavonoids: An overview. Int J Pharm Sci Res. 2019;10:1567–74. DOI: 10.13040/IJPSR.0975-8232.10(4).1567-74
  6. Kaurinovic B, Vastag D. Flavonoids and phenolic acids as potential natural antioxidants. Antioxidants, IntechOpen London, UK. 2019;1–20. DOI: 10.5772/intechopen.83731
  7. Daré RG, Nakamura CV, Ximenes VF, et al. Tannic acid, a promising anti-photoaging agent: Evidences of its antioxidant and anti-wrinkle potentials, and its ability to prevent photodamage and MMP-1 expression in L929 fibroblasts exposed to UVB. Free Radic Biol Med. 2020;160:342–55. DOI: 10.1016/j.freeradbiomed.2020.08.019
  8. Tena N, Martín J, Asuero AG. State of the art of anthocyanins: Antioxidant activity, sources, bioavailability, and therapeutic effect in human health. Antioxidants. 2020;9:451. DOI: 10.3390/antiox9050451
  9. Zingare ML, Zingare PL, Dubey AK, et al. Clitoria ternatea (Aparajita): A review of the antioxidant, antidiabetic and hepatoprotective potentials. Int J Pharm Biol Sci. 2013;3(1):203–213.
  10. Puspitasari D, Pratimasari D, Andriani D. Penentuan nilai SPF (sun protective factor) krim ekstrak etanol bunga telang (Clitoria ternatea) secara in vitro menggunakan metode spektrofotometri. J Insan Farm Indones. 2019;2(1):118–125.
  11. Pittayapruek P, Meephansan J, Prapapan O, et al. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci. 2016;17:868. DOI: 10.3390/ijms17060868
  12. Pandel R, Poljsak B, Godic A, et al. Skin photoaging and the role of antioxidants in its prevention. ISRN Dermatology. 2013;11:930164. DOI: 10.1155/2013/930164
  13. Fisher GJ, Quan T, Purohit T, et al. Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin. Am J Pathol. 2009;174:101–14. DOI: 10.2353/ajpath.2009.080599
  14. Duarte J, Francisco V, Perez-Vizcaino F. Modulation of nitric oxide by flavonoids. Food Funct. 2014;5:1653–68. DOI: 10.1039/c4fo00144c
  15. Wiraguna AAGP, Dianasari R, Pangkahila W. The topical skin application of purple corn extract (Zea mays) inhibited the increase in MMP-1 levels and decreased collagen in wistar rats (Rattus norvegicus) exposed to UV-B rays. Biomed Pharmacol J. 2019;35:297–304. DOI: 10.13005/bpj/1641