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Background: UV-B irradiation is the major causative factor in photoaging. Photoaging increases MMP-1 significantly and cause damage to collagen by forming ROS. The monounsaturated free fatty acids and antioxidant compounds in Tamanu oil have been studied to inhibit the emergence of MMP-1 and maintain collagen amount. The study aimed to investigate topical Tamanu oil in inhibiting MMP-1 expressions and collagen decrease in male Wistar rats’ skin exposed to UV-B.

Methods: A randomized post-test-only control group design was performed on 36 male Wistar rats, aged 2-3 months old with 180-220 grams body weight. They were assigned to two groups: the placebo group and the Tamanu oil group. Both groups were given the same amount of UV-B exposure for four weeks totalling in 840 mJ/cm2 of dosage radiation. Their dermis was examined for MMP-1 using Immunohistochemistry and collagen amount using Picro Sirius Red staining.

Results: A comparative analysis was done to show the difference between groups. The mean expression of MMP-1 in the control group was 34,03±2.97 cell/HPF and the Tamanu oil group was lower at 22.73±2.68 cell/HPF. The mean amount of collagen dermis in the control group was 38.90±11.64 % and the Tamanu oil group was higher at 65.66±8.13 %. A significant difference was shown in the two groups (p<0.05).

Conclusion: Topical Tamanu oil has proven to hinder the increase of MMP-1 expressions and collagen amount in male Wistar rats’ skin exposed to UV-B.


Matrix Metalloproteinase 1 Calophyllum Skin Aging Reactive Oxygen Species Ultraviolet Rays Collagen

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How to Cite
Gunawan, Y., Pangkahila, A., & Darwinata, A. E. (2021). Topical administration of Tamanu Oil (Calophyllum inophyllum) inhibited the increase of matrix metalloproteinase-1 (MMP-1) expressions and decrease of collagen dermis amount in male wistar rats exposed to ultraviolet B. Neurologico Spinale Medico Chirurgico, 4(3).


  1. Wiraguna AAGP. Aspek anatomi dan fisiologi kulit. 1st ed. Denpasar: Udayana University Press; 2020.
  2. Chiang HM, Chen HC, Lin TJ, Shih IC, Wen KC. Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts. Food Chem Toxicol. 2012;50(12):4260-4269. DOI:10.1016/j.fct.2012.08.018
  3. Young AR, Claveau J, Rossi AB. Ultraviolet radiation and the skin: Photobiology and sunscreen photoprotection. J Am Acad Dermatol. 2017;76(3S1):S100-S109. DOI:10.1016/j.jaad.2016.09.038
  4. Ansel JL, Lupo E, Mijouin L, et al. Biological activity of Polynesian Calophyllum inophyllum oil extract on human skin cells. Planta Medica. 2016;82:961-966. DOI: 10.1055/s-0042-108205
  5. Susanto DF, Aparamarta HW, Widjaja A, et al. Calophyllum inophyllum: Beneficial phytochemicals, their uses, and identification. Phytochemicals in Human Health. 2019. IntechOpen. DOI: 10.5772/intechopen.86991
  6. Raharivelomanana P, Ansel JL, Lupo E, et al. Tamanu oil and skin active properties: from traditional to modern cosmetic uses. OCL. 2018;Vol.25:5. DOI: 10.1051/ocl/2018048
  7. Wang F, Wu J. Modulating effect of fatty acids and sterols on skin aging. Journal of Functional Foods. 2019;57:135-140. DOI: 10.1016/J.JFF.2019.04.011
  8. Kaurinovic B, Vastag D. Flavonoids and phenolic acids as potential natural antioxidants. Antioxidants, IntechOpen London, UK. 2019;1–20. DOI: 10.5772/intechopen.83731
  9. Rejeki A. Ekstraksi dan penetapan nilai SPF minyak Nyamplung dengan metode sektrofotometri (extraction and SPF value determination of Tamanu oil by spectrofotometri method). IJMS – Indonesian Journal in Medical Science. 2015;Vol.2(1);7-10.
  10. Leu T. Contribution à la connaissance de la flore polynésienne: évaluation de l’intérêt pharmacologique de quelques plantes médicinales et étude phytochimique du Tamanu (Calophyllum inophyllum L. – Clusiaceae). The Hallmarks of Aging. Cell. 2009;153(6):1194-1217.
  11. Kwon KR, Alam M., Park JH, et al. 2019. Attenuation of UVB-induced photo-aging by polyphenolic-rich Spatholobus suberectus stem extract via modulation of MAPK/AP-1/MMPs signaling in human keratinocytes. MDPI Nutrients, 2019;11:1341. DOI: 10.3390/nu11061341
  12. Pittayapruek P, Meephansan J, Prapapan O, et al. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci. 2016;17:868. DOI: 10.3390/ijms17060868
  13. Quan T, Fisher GJ. Role of age-associated alterations of the dermal extracellular matrix microenvironment in human skin aging: A mini-review. Gerontology. 2015;61(5):427–434. DOI: 10.1159/000371708
  14. Sarkar R, Podder I, Gokhale N, et al. Use of Vegetable oils in dermatology: An overview. International Journal of Dermatology. 2017;56(11). DOI: 10.1111/ijd.13623
  15. Lin TK, Zhong L, Santiago JL. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. Int J Mol Sci. 2017;19(1):70. DOI: 10.3390/ijms19010070
  16. Dzialo M, Mierziak J, Korzun U, et al. The potential of plant phenolics in prevention and therapy of skin disorders. Int J Mol Sci. 2016;17(2):160. DOI: 10.3390/ijms17020160
  17. Bangun DY, Aman IGM, Winarti, NW. Topical Argan oil inhibited prostaglandin E2 and prevented collagen reduction in male wistar rat’s skin exposed to UVB. Biochemistry Science. 2021;10:(8)779-782. DOI: 10.21275/SR21816082942
  18. Sugianto M, Achadiyani, Nugraha GI. Antioxidant effects of Red Fruit oil on MMP-1 gene expression and malondialdehyde levels on skin exposed to UVB rays. Mol Cell Biomed Sci. 2019; 3(2): 100-6. DOI: 10.21705/mcbs.v3i2.68
  19. Lohan SB, Vitt K, Scholz P, Keck CM, Meinke MC. ROS production and glutathione response in keratinocytes after application of β-carotene and VIS/NIR irradiation. Chem Biol Interact. 2018; 280: 1-7. DOI: 10.1016/j.cbi.2017.12.002