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Background: The primary acute damage due to UV radiation is keratinocyte apoptosis or sunburn cell. Previous studies showed that antioxidants inhibit UV damage. Java plum (Syzygium cumini) which is found in Indonesia, was known to have a high concentration of antioxidants. This study was aimed to assess the Java plum ethanol extract cream effects on the number of sunburn cells on the dorsal skin of male Wistar rats that exposed to UV-B.
Methods This experimental research was a post-test only control group design that was conducted on 30 male rats. Groups were divided into control, placebo, 5%, 10%, and 20% ethanol extract cream concentrations (P1, P2, P3, P4, and P5, respectively). The number of sunburn cell were then observed and recorded based on visual evaluation of the skin biopsy under a light microscope.
Results: The mean results of P1, P2, P3, P4, and P5 were 132.67, 98.67, 118.67, 104.00, and 54.67 respectively. The control, placebo, 5%, and 10% ethanol extract cream showed no significant result (p>0.05). In contrast, 20% ethanol extract cream showed a protective effect (p<0.05).
Conclusion: Java plum ethanol extract cream with 20% dose could decrease the number of sunburn cells in the skin.
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Zhang S, Duan E. Fighting against skin aging. Cell Transplantation. 2018;27(5):729-38. DOI: 10.1177/0963689717725755
- Aji A, Erwinda E, Yusrawati Y, et al. Vitamin D deficiency status and its related risk factors during early pregnancy: a cross-sectional study of pregnant Minangkabau women, Indonesia. BMC Pregnancy and Childbirth. 2019;19(1):183. DOI: 10.1186/s12884-019-2341-4
- D'Orazio J, Jarrett S, Amaro-Ortiz A, et al. UV radiation and the skin. International Journal of Molecular Sciences. 2013;14(6):12222-48. DOI: 10.3390/ijms140612222
- Craig S, Earnshaw C, Virós A. Ultraviolet light and melanoma. The Journal of Pathology. 2018;244(5):578-85. DOI: 10.1002/path.5039
- Mueller J, Heckathorn S, Fernando D. Identification of a chloroplast dehydrin in leaves of mature plants. International Journal of Plant Sciences. 2003;164(4):535-42. DOI: 10.1086/375376
- Do Carmo Brito B, da Silva Pena R, Santos Lopes A, et al. Anthocyanins of jambolão (Syzygium cumini): extraction and pH-dependent color changes. Journal of Food Science. 2017; 82(10):2286-90. DOI: 10.1111/1750-3841.13847
- Ayyanar M, Subash-Babu P. Syzygium cumini (L.) skeels: A review of its phytochemical constituents and traditional uses. Asian Pacific Journal of Tropical Biomedicine. 2012;2(3):240-6. DOI: 10.1016/s2221-1691(12)60050-1
- Krismayogi G, Ratnayanti I, Linawati N, et al. Purple cabbage extract cream effect on erythema score of male Wistar rats back skin exposed to UV-B radiation. Biomedical and Pharmacology Journal. 2018;11(1):343-51. DOI: 10.13005/bpj/1378
- Dunaway S, Odin R, Zhou L, et al. Natural antioxidants: Multiple mechanisms to protect skin from solar radiation. Frontiers in Pharmacology. 2018;9:392. DOI: 10.3389/fphar.2018.00392
- Mnich C, Hoek K, Virkki L, et al. Green tea extract reduces induction of p53 and apoptosis in UVB-irradiated human skin independent of transcriptional controls. Experimental Dermatology. 2009;18(1):69-77. DOI: 10.1111/j.1600-0625.2008.00765.x
- Olfa P, Vidrighinescu R, Petruta A, et al. Photoprotective effects of Romanian propolis on skin of mice exposed to UVB irradiation. Food and Chemical Toxicology. 2013;62:329-42. DOI: 10.1016/j.fct.2013.08.078
- Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatology Research and Practice. 2012;2012:1-4. DOI: 10.1155/2012/135206
- Afaq F, Syed D, Malik A, et al. Delphinidin, an anthocyanidin in pigmented fruits and vegetables, protects human hacat keratinocytes and mouse skin against UVB-mediated oxidative stress and apoptosis. Journal of Investigative Dermatology. 2007;127(1):222-32. DOI: 10.1038/sj.jid.5700510
- Merzlyak M, Chivkunova O, Solovchenko A, et al. Light absorption by anthocyanins in juvenile, stressed, and senescing leaves. Journal of Experimental Botany. 2008;59(14):3903-11. DOI: 10.1093/jxb/ern230
- Cimino F, Ambra R, Canali R, et al. Effect of cyanidin-3-o-glucoside on UVB-induced response in human keratinocytes. Journal of Agricultural and Food Chemistry. 2006;54(11):4041-47. DOI: 10.1021/jf060253x